Page 94 - D. Cancer biology

[D. Cancer biology-62]

Inhibition of LPA receptor 2 induces cell death of ovarian

cancer stem cells

Min Joo Shin¹, Dae Kyoung Kim¹, Seo Yul Lee¹, Ye Eun Kim¹, Jae Ho Kim¹

¹Department of Physiology, Pusan National University, Yangsan 50612, Republic of Korea

Cancer stem cells(CSCs) are a subpopulation of cancer cells characterized by self-renewal ability, tumorigenesis and
drug resistance. In previous studies, we have shown that autotaxin and lysophosphatidic acid (LPA) play an important
role in CSCs characterization such as migration, sphere forming ability and drug resistance in ovarian cancer. So in

this study, we found a drug that could target CSCs by inhibiting the LPA pathway. When the LPA inhibitor was

treated with A2780-SP, sphere-forming ability was significantly reduced compared to the treatment with KI16425
and Paclitaxel. When ovarian CSCs and paclitaxel were treated with ovarian cancer cells, cancer cells showed low
survival rate even at low concentration and CSCs showed high survival rate even at high concentration. However,

when LPA inhibitor was treated with CSCs and ovarian cancer cells, survival rate was much lower in cancer stem
cells than in cancer cells. It was confirmed that ovarian CSCs isolated from ovarian cancer patients had the same

results as cell lines. It was confirmed that the cells were killed more by the lpa inhibitor treatment as in the previous
results. These results suggest that the LPA inhibitor may be an effective drug for the treatment of ovarian cancer

stem cells.