Page 18 - D. Cancer biology

[D. Cancer biology-14]

Anti-cancer Effects of β-apopicropodophyllin Against

Colorectal Cancer Cells

Na-Gyeong Lee¹˙², A-RAM Kang², Jin-Hee Kwon², Hong-Duck Um², Joon Kim¹˙*, Jong Kuk Park²˙*

¹Department of Life Sciences, Korea University, Seoul 02841, Korea, ²Division of Applied Radiation Bioscience,

Korea Institute of Radiological & Medical Sciences, Seoul 01812, Korea

In previously study, we have isolated podophyllotoxin acetate (PA), a naturally occurring derivative of
podophyllotoxin (PPT), from Natural Product Library. PA had anti-cancer activities against one of the non-small cell

lung cancer (NSCLC) cell lines, but is physiologically unstable for therapeutic drug and contains highly toxicity. For

that, we synthesized several new derivatives of PPT and identified β-apopicropodophyllin (APP) as a potential cell
death inducer. In this study, we tested whether APP has anti-cancer drug effect against colorectal cancer. Colorectal
cancer (CRC) is the second most common cancer in women and the third most common in men. First, we examined

whether APP could act as a radiosensitizer in CRC cells. APP additionally showed anti-cancer activity against HCT116
and DLD-1 cell lines with IC50 values of 7.877nM and 8.219nM, respectively. Annexin V-Propidium iodide assay

indicated a combination of APP and γ-ionizing radiation (IR) that increased apoptotic cell death. We also performed
H2DCFDA-based assay, and then observed APP and IR co-treatment increased ROS levels in CRC cells. Taken

together, co-treatment of APP and IR might promote increase of cell death followed by ROS induction in CRC cells.