Page 12 - C. Biotechnology and molecular imaging

[C. Biotechnology and molecular imaging-8]

[Part 1] Comparison of Clearing Efficacies of Various Tissue

Clearance Protocols in Rodent Brain

Jiwon Woo¹˙²˙, Mirae Lee¹˙², Jeong-Yoon Park¹˙², Yong-Eun Cho¹˙²˙*

¹Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul 03722, Republic of Korea, ²The Spine

and Spinal Cord Institute, Department of Neurosurgery, Gangnam Severance Hospital, Yonsei University College of
Medicine, Seoul 06273, Republic of Korea

The mammalian biological systems such as the central nervous system including brain and spinal cord consist of

over thousands of distinct cell types forming widely interconnected functional neural networks, and coordinated

across multiple size scales, from the sub-nanoscale of molecules to the macroscale, the tissue wide interconnectivity
of the cell populations. Here we introduce comparative analyses of clearing efficacies in various tissue clearing
techniques for high resolution imaging analysis of the multiscale organization of intact brain tissues in rodent. We

evaluated clearing efficacies of the brain tissue using improved methods with twenty passive clarity methods that
allowed production of transparent brain by tissue clearing. The tissue comparative analysis facilitates the rapid

examination of three dimension morphological and therapeutic aspects of surgical animal disease models and can
be selective used to aid in the investigating of medical and experimental conditions. Our comparative analyses of

tissue clearing efficacies suggest that useful to provide access to adaptable method for stereoscopic multi-scale
information that will expand current understanding of health and disease.