Page 10 - C. Biotechnology and molecular imaging

[C. Biotechnology and molecular imaging-7]

Optimization of the serotype a foot and mouth disease

inactivation vaccine antigen production using pilot-scale

equipment

Jae-Seok Kim¹, Sang Hyun Park¹, Hyejin Kim¹, Ah-Young Kim¹, Sun Young Park¹, Na Young Kang¹,

Jong-Hyeon Park¹, Young-Joon Ko¹˙*

¹Center for FMD Vaccine Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea

Foot-and-mouth disease (FMD) is the most severe livestock disease due to the economic consequences of its

outbreak by infect cloven-hoofed animals. FMD virus (FMDV), belongs to the Aphthovirus genus of the family
Picornaviridae. The FMDV rapidly replicates and spreads within the infected animal, among in-contact susceptible

animals, and by aerosol. Korea has maintained a vaccination policy and imported FMD vaccines from foreign
manufacturers. Despite the vaccination policy of the government, FMD occurred continuously since 2014. Due to

the unstable supply of imported vaccines and the rising demand for customized vaccines, R&D is under progress
to produce the FMD vaccine using local strains. In this study, we established the antigen production process of

FMDV type A Yeoncheon with pilot-scale. To develop a FMD vaccine, we optimized the culture condition of the type
A Yeoncheon virus on a flask scale. A preliminary test was carried out in small-scale to find optimal conditions to

yield the maximum recovery of virus antigen. Based on the optimized conditions at the flask level, we performed
the test using a bioreactor, resulting 2 ug/ml 146S antigen at virus supernatant in a 100L scale. We expect that this

result would be the groundwork for FMD vaccine manufacturing in Korea.