Page 29 - A. Aging

Evaluation of inflammation, wound healing, and skin irritation by DM seed
oil in vitro human skin cells and on reconstructed human epidermis
Eunsu Song , Jaeyoung Choi , Kyo-Yeon Lee , Sung-Gil Choi , Yun Hee Chang , Jinah Hwang 1
1
1
1
2
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1 Food and Nutrition, Myongji University, Yongin, Republic of Korea, Food Science and Technology, Gyeongsang National University, Jinju, Republic of Korea
BACKGROUND AIM
Dracocephalum moldavica L. (DM), has been consumed Since cosmetic animal testing has been banned in
as alternative medicine due to its various functions such many countries, the importance of alternative methods
as anti-oxidant and anti-inflammatory effects from its bio- to animal testing has emerged. This study was carried
active compounds. Particularly, DM seed oil is rich in ω-3 out to investigate possibility of DM as pharmaceutical
which may play a critical role in anti-inflammation. agent using in vitro alternative testing method.

METHODS

I II III

Skin-reconstructed
human epidermis
HDF HaCaT HDF HaCaT
mRNA expression of Cytotoxicity on skin-reconstructed
DM seed oil inflammatory cytokines Wound healing assay
Dracocephalum moldavica L. by supercritical fluid extraction human epidermis model
RESULTS

DM seed oil (DMS) suppressed mRNA (A) HDF (B) HDF Figure 2. Effect of DMS on cell
levels of aging- and inflammatory-related migration in human dermal
cytokines such as IL-6, IL-8, and COX-2 in cells. (A), (B) representative
dose-dependent manner. Furthermore, images and Image J analysis
DMS enhanced the speed of wound of wound closure (%) in HDF
healing in two types of skin cells showing (C), (D) representative images
different cell migration rate according to (C) HaCaT (D) HaCaT and Image J analysis of
wound closure (%) in HaCaT
cell types. In skin irritation test on RHE, all (n=3). EGF, epidermal growth
doses (0.25-8 %) of DM seed oil increased factor; SC-oil, oil extraction by
cell viability compared to positive control supercritical fluid CO 2 extract of
(SDS). Dracocephalum moldavica L
seed.
(A) HDF (B) HaCaT
(A) RHE model (B) Cell toxicity (C) IL-6 level in the RHE medium
5 % SDS DPBS
0.25 % DMS 0. 5 % DMS
1 % DMS 2 % DMS
4 % DMS 8 % DMS

Figure 3. Skin irritation test of DMS on skin reconstructed human epidermis model
Figure 1. DMS reduced mRNA level of inflammatory (RHE). (A) RHE on 24 well plate after MTT solution treatment (B) cell toxicity rate of
cytokines in skin cells (n=3). (A) HDF (B) HaCaT RA, DMS (C) the level of IL-6 in the medium of RHE samples (n=3).
retinoic acid; DMS, extraction by supercritical fluid CO 2 SDS, sodium dodecyl sulfate; DPBS, Dulbecco's Phosphate Buffered Saline; DMS, extraction
extract of Dracocephalum moldavica L seed. by supercritical fluid CO 2 extract of Dracocephalum moldavica L seed.

CONCLUSION REFERENCES ACKNOWLEDGEMENTS

DMS ameliorates inflammatory - Lee et al., Disease-specific primed This work was supported under the
cytokines which may affect aging human adult stem cells effectively framework of international cooperation
process and showed lower toxicity ameliorate experimental atopic dermatitis program managed by the National
Research
Foundation
of
Korea
on skin reconstructed epidermis in mice, Theranostics 2019; 9(12):3608- (2017R1A2B4010140).
model. In conclusion, DMS may be 3621
-Motoyama et al., Anti-allergic effects of
potentially applied to recovery anti- novel sulfated polysaccharide sacran on CONTACT INFORMATION
aging cosmetic products as mouse model of 2,4-Dinitro-1- Eunsu Song: eunsu4979@gmail.com
important ingredient due to its fluorobenzene -induced atopic dermatitis,
faster wound closure ability and International Journal of Biological Jinah Hwang*: jhwang@mju.ac.kr
less skin irritation. Macromolecules, 108, 112-118 *corresponding author