Page 104 - ebook

[A. Biochemistry/Molecular Biology] A-52

Perilla heading date gene, PfHd3a, regulate flowering time in

short day condition

Jee Hye Kim¹, Jae Bok Heo¹*

¹Department of Molecular Biotechnology, Dong-A University, Pusan 49315, Korea

Perilla (Perilla frutescens) is an annual plant and grown in Asian countries including Korea, Japan, China. Although
perilla is mainly used to seed and leaf for food or industrial materials, much is unknown about research of perilla.

Previously, we found a gene in RNA-seq results which may play an important role in flowering time, and it was
identified to be an ortholog of OsHd3a in rice. Hd3a is well known boost to heading under short-day (SD) conditions.

In this study, we focused on this pfHd3a. Since tissue culture system using agrobacterium was developed, we made
a pfHd3a-overexpressing plant in perilla using agrobacterium-mediated transformation. Agrobacterium strain

EHA105 harboring constructs pB2GW7-pfHd3a containing phosphinothricin (PPT) was used for genetic
transformation. A reproducible shoot was induced from hypocotyl explants on MS basal medium supplemented

with 3.0 mg/l 6-Benzylaminopurine (BA), 0.01mg/l indole-3-acetic acid (IAA), 250mg/l cefotaxime, 500mg/l
carbenicillin and 1.2 mg/l PPT and candidates were selected. Rooting was induced on half-strength hormone-free

MS medium. The transformants were confirmed by PCR of PPT resistance region. Next generation seeds were used
for flowering phenotype check compared wild type under daylength condition.