Page 4 - R. Organoid

[R. Organoid-2]

Comparative proteomic analysis and evaluation of anticancer

drug responses using patient-derived tumor organoids

Yeon-Jin Chu¹˙#, Hyuna Kim¹˙#, Hyeong Ho Cho¹, Ki Soon Kim¹, Donghyun Park¹, Woo Yong Oh¹,
Yoonsook Lee¹˙*

¹Clinical Research Division, National Institute of Food and Drug Safety Evaluation, Osong Health Technology

Administration Comple, Cheongju 28159, Korea

Tumor organoids using 3D culture system are well known to reflect the heterogeneity and biological properties of
patients compared with cancer cell lines using 2D culture system. However, there are few studies of cancer

mechanism and drug effects using tumor organoids. In this study, we investigated the effects and the alteration of
proteins by anticancer drug using patient-derived tumor organoids. At first, we cultured patient-derived tumor

organoids isolated from colorectal cancer speciemens using matrigel. Each tumor organoids were shown different
growth properties and proteins expression level. Then, we evaluated the cytotoxicity of 5-Fluorouracil(5-FU) in tumor

organoids. We found that 5-FU induced cell death was caused by apoptosis through the expression of apoptotic
proteins. Based on these data, we tried to find proteins associated with 5-FU resistance using 2-dimensional gel

eletrophoresis(2-DE) and MALDI-TOF. Most of proteins were involved in metabolism, synthesis/degradation of
protein, cytoskeleton, redox reaction, etc. We measured mRNA and protein levels of them, and selected candidates

for biomarker of 5-FU resistance. Our data shows that tumor organoids can be useful tool of tumor studies for
evaluation and investigation of anti-cancer drug.