Page 28 - M. Immunology

[M. Immunology-20]

Therapeutic and anti-inflammatory effect of Bleomycin

induced Systemic sclerosis using W-peptide

Gyutae Park¹, Yangwoo Kwon¹, Young-cheol Song¹, Jaeho Kim¹˙*

¹Physiology, Pusan National University School of Medicine, Yangsan 50612, Korea

Systemic sclerosis is an autoimmune disease that affects multiple organs. It is caused by irregulated immune function
and enlargement of connective tissue. Abnormal inflammatory activation is induced by inflammatory cell activation
and cytokine expression, that induce fibrosis, such as fibroblast activation, and collagen production. Formyl Peptide

Receptor-2(FPR2) is a G-protein coupled receptor that expressed in Neutrophil, and Monocyte. It is regulate

inflammatory cell apoptosis, and inflammatory cytokine expression. In previous reports was conducted to investigate
the effect of FPR2 activation in sclerosis. So, We focused on FPR2 activation in scleroderma model for anti-
inflammation. So, we selected W-peptide administration, a synthetic peptide of FPR2 agonist, in bleomycin induced

scleroderma mouse model. So, W-peptide have therapeutic effect in scleroderma model, through dermal thickness
distance and fibrosis regulation, and inhibit inflammatory cell infiltration and cytokine expression. However, W-

peptide mediated scleroderma therapeutic effects are abrogated in FPR2 knockout mice. These results suggests
that FPR2 agonistic W-peptide ameliorates fibrosis by stimulation inflammatory regulation in scleroderma model.