Page 55 - F. Cell biology

Targeting both apoptosis and necroptosis by resveratrol and docetaxel
induces synergistic cytotoxicity of prostate carcinoma cells
Yoon-Jin Lee and Sang-Han Lee

Department of Biochemistry, College of Medicine, Soonchunhyang University, Cheonan 31151, Republic of Korea

BACKGROUND AIM

Androgen deprivation therapy is one of the main treatment The present study was performed to investigate the
options for patients with early-stage androgen-dependent efficacy of resveratrol during combination treatment
prostate cancer, but almost all patients proceed to develop with the anticancer drug docetaxel in prostate
androgen-independent prostate cancer in a few years. carcinoma LNCaP cells, including details of factors
Progress to an incurable and irreversible androgen- related to detailed cell death mechanisms.
independent stage remains a major obstacle to effective
treatment, and a strong need for alternative treatment
strategies is being emphasized.

METHODS

1. Cell lines: Prostate cancer LNCaP cells 2. Cell viability assay: MTT assay
3. Annexin V-PE binding assay 4. Cell cycle analysis
5. Western blot analysis 6. Measurement of intracellular ROS
7. Spheroid culture and viability assay 8. Spheroid staining
9. Statistical analysis

RESULTS

CONCLUSION REFERENCES ACKNOWLEDGEMENTS

We report resveratrol as an adjuvant 1. Ko JH, Sethi G, Um JY, Shanmugam MK, This research was supported by the Basic
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mechanisms of necroptosis should be Hattori K, Zhao QL, Suzuki T, Noguchi M, Ohashi
W, Hattori Y. Different effect of resveratrol to Contact information
investigated comprehensively, induction of apoptosis depending on the type of
simultaneously targeting apoptosis and human cancer cells. Int J Oncol 2017;50:787-97. Corresponding Author: Yoon-Jin Lee,
necroptosis in cancer therapy might 3. Hu S, Li X, Xu R, Ye L, Kong H, Zeng X, Wang Tel, 82-41-570-2440, Fax, 82-41-579-
provide very promising opportunities for H, Xie W. The synergistic effect of resveratrol in 6080, Email, leeyj@sch.ac.kr
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