Page 52 - F. Cell biology

[F. Cell biology-30]

Cell death according to silica nanoparticles manufactured by

different methods

Jin Ee Baek¹, Jae Hoon Shin¹, Jong Seong Lee¹,

¹Pathogenic laboratory research, Institute Of Occupation And Environment, Incheon 21417, Korea

Silica nanoparticles can be roughly divided into two types: wet route base synthesis method and thermal route base
(Pyrogenic) synthesis method. Differences in the silica production method may affect the physicochemical properties
of silica nanoparticles, and may cause different cytotoxicity results for silica nanoparticles. However, there have been

few studies on the cytotoxicity of silica nanoparticles made by other methods. The purpose of this study was to

determine the difference in cytotoxicity and cell death mechanism according to the physicochemical properties of
silica nanoparticles by confirming the difference in cytotoxicity according to the method of manufacturing silica
nanoparticles. After diluting the silica dispersed in the culture medium to A549 cells cultured for 24 hours, and

putting them in cells to a concentration of 0-5000 ug/ml, reacting for 24 hours. As a result, viability of cells exposed
to silica nanoparticles made by wet pretreatment and cells exposed to fumed silica nanoparticles showed a

difference, and LDH secretion and ROS generation due to cell damage also showed different results. In conclusion,
differences in the production method of silica nanoparticles can affect the cytotoxicity and cell death mechanism.