Page 82 - D. Cancer biology

[D. Cancer biology-55]

Secreted kinase Fam20C regulates extracellular

phosphoproteome that related to glioblastoma recurrence

Chan Il Kim¹, Jong Bae Park¹

¹Cancer Biomedical Science, Graduate School of Cancer Science and Policy, National Cancer Center, Goyang

10408, Korea

Glioblastoma multiforme (GBM) is one of the most aggressive primary brain tumors. Advancements in therapeutics
during the past decades have not significantly increased the overall survival of patients with this disease. Although

recent approaches slightly increased progression free survival, recurrence of GBM seems to be inevitable and its

therapeutic outcome is highly case dependent. Therefore, we postulate that during this progression free period,
GBM cells acquire drug resistance. Through the proteomic analysis in well-established avastin resistance mouse
model, we identified that a large number of protein expression signatures are altered. Among those proteins, Family

with sequence similarity 20 member C (Fam20C) is overexpressed in recurrence tumor tissue induced by avastin.
Fam20C is a kinase that phosphorylates S-x-E/pS motifs on proteins in the extracellular matrix, extracellular domain

of membrane protein and other secreted protein. Fam20C generates the majority of the extracellular
phosphoproteome. After Fam20C overexpression, we confirmed that recurrence tumor grows rapidly. In this study,

we show one of Fam20C targets phosphorylation signaling cascade that related to tumor recurrence.