Page 50 - D. Cancer biology
P. 50
[D. Cancer biology-38]
Dehydroabietic acid induces cell cycle arrest and apoptosis in
gastric cancer
Won-Jin Kim¹˙², Hana Lee¹˙², Seok-Jun Kim¹˙²˙*
¹Department of Integrative Biological Science, Chosun university, Gwangju 61452, Republic of Korea, ²BK21-Plus
Research Team for Bioactive Control Technology, Chosun university, Gwangju 61452, Republic of Korea
Gastric cancer is one of the serious diseases with a high incidence worldwide. Chemotherapy and radiation therapy
are commonly used to treat gastric cancer. Conventional anti-cancer drugs have limited effectiveness. Therefore,
the development of new anti-cancer drugs is important. Dehydroabietic acid (DAA) is a type of diterpene and known
to have anti-bacterial, anti-inflammatory and anti-cancer effects. However, the effects on human gastric cancer cell
lines have not been reported much. In this study, we examined the effects of DAA on cell growth inhibition in
human gastric cancer cell lines. The expression of Bax known as pro-apoptotic protein was up-regulated, Bcl-2
known as anti-apoptotic protein was down-regulated in DAA treated AGS cells. Moreover, induce cleavage of
Caspase-9 and –3, Poly (ADP-ribose) polymerase (PARP). In addition, the expression of cyclin D1, Cdk2, 4 related to
G1 phase progression was also decreased. These results suggest that DAA induced cell death though the apoptosis
& G1 cell cycle arrest. Additionally, we performed RNA sequencing. Various genes related to Apoptosis and cell
cycle were changed by DAA treatment in AGS cells. These results suggest that DAA is a potent drug that suppresses
the growth of gastric cancer cells.
