Page 49 - D. Cancer biology

Dehydroabietic acid induces cell cycle arrest and apoptosis in gastric cancer

Won-Jin Kim, Hana Lee and Seok-Jun Kim

Department of Integrative Biological Science, Chosun university, Gwangju 61452, Republic of Korea,
BK21-Plus Research Team for Bioactive Control Technology, Chosun university, Gwangju 61452, Republic of Korea
ABSTRACT

Gastric cancer is one of the serious diseases with a high incidence worldwide. Chemotherapy and radiation therapy are commonly used to treat
gastric cancer. Conventional anti-cancer drugs have limited effectiveness. Therefore, the development of new anti-cancer drugs is important.
Dehydroabietic acid (DAA) is a type of diterpene and known to have anti-bacterial, anti-inflammatory and anti-cancer effects. However, the
effects on human gastric cancer cell lines have not been reported much. In this study, we examined the effects of DAA on cell growth inhibition
in human gastric cancer cell lines. The expression of Bax known as pro-apoptotic protein was up-regulated, Bcl-2 known as anti-apoptotic
protein was down-regulated in DAA treated AGS cells. Moreover, induce cleavage of Caspase-3 and , Poly (ADP-ribose) polymerase (PARP).
In addition, the expression of cyclin D1, Cdk2, 4 related to G1 phase progression was also decreased. These results suggest that DAA induced
cell death though the apoptosis & G1 cell cycle arrest. Additionally, we performed RNA sequencing. Various genes related to Apoptosis and
cell cycle were changed by DAA treatment in AGS cells. These results suggest that DAA is a potent drug that suppresses the growth of gastric
cancer cells.

RESULTS

CONCLUSION REFERENCES ACKNOWLEDGEMENTS

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regulated by DAA. These results molecular sciences 19.2 (2018): 448. E-mail address: wjsh003@naver.com
suggest that DAA regulates the
expression of various genes that
regulate apoptosis and the cell cycle.