Page 102 - D. Cancer biology

[D. Cancer biology-66]

Leucyl-tRNA Synthetase Regulates Cell Proliferation and

Invasion in Human Colorectal Cancer Cells

Sang-Heum Han¹˙², Su Jeong Park¹, Jun Gi Cho¹, Joo-In Park¹˙²˙*

¹Department of Biochemistry, Dong-A University College of Medicine, Busan 49201, South Korea, ²Peripheral

Neuropathy Research Center, Dong-A University, Busan 49201, South Korea

Leucyl-tRNA synthetase (LARS) senses intracellular leucine and activates mechanistic target of rapamycin complex
1 (mTORC1). Several studies suggest that LARS can be a potential target of anticancer agents. However, the detailed

molecular mechanisms of LARS on the cell proliferation and invasion in human colorectal cancer cells are clearly

undefined. In this study, to clarify the molecular mechanisms of LARS actions in tumor progression, we established
LARS-overexpressing stable SW480 cells and LARS-knocked down stable SW620 cells, and then performed cell
proliferation, migration and transwell invasion assay. LARS overexpression enhances cell proliferation, migration and

invasion, and LARS knockdown reduces them. We examined the expression of several molecules related with cell
proliferation and invasion by western blot analysis. Expression of cyclin D1, c-Myc, and vimentin was decreased by

LARS knockdown. These results indicate that cyclin D1, c-Myc, and vimentin might contribute to regulation of cell
proliferation and invasion by LARS. Further studies to investigate the molecular network between c-Myc and LARS

in human colorectal cancer cells are needed. This study was supported by the NRF funded by the Korean
Government (MSIP) (No. 2016R1A5A2007009).