Page 18 - C. Biotechnology and molecular imaging

[C. Biotechnology and molecular imaging-11]

[Part 4] A Cell-MAP Method for Super-Resolution Cell

Imaging that Retains Fluorescence

Jiwon Woo¹˙²˙#, Mirae Lee¹˙²˙#, Jeong-Yoon Park¹˙²˙*

¹Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul 03722, Republic of Korea, ²The Spine

and Spinal Cord Institute, Department of Neurosurgery, Gangnam Severance Hospital, Yonsei University College of
Medicine, Seoul 06273, Republic of Korea

Biological systems consist of a variety of distinct cell types that form functional networks. Super-resolution imaging

of individual cells is required for better understanding of these complex systems. Direct visualization of 3D

subcellular and nano-scale structures in cells is helpful for the interpretation of biological interactions and system-
level responses. Here we introduce a modified magnified analysis of proteome (MAP) method for cell super-
resolution imaging (Cell-MAP) which preserves cell fluorescence. Cell-MAP expands cells more than four-fold while

preserving their overall architecture and three-dimensional proteome organization after hydrogel embedding. In
addition, Optimized-Cell-MAP completely preserves fluorescence and successfully allows for the observation of

tagged small molecular probes containing peptides and microRNAs. Optimized-Cell-MAP further successfully
applies to the study of structural characteristics and the identification of small molecules and organelles in

mammalian cells. These results may give rise to many other applications related to the structural and molecular
analysis of smaller assembled biological systems.