Page 22 - C. Biotechnology and molecular imaging

[C. Biotechnology and molecular imaging-13]

Canine amniotic membrane-derived mesenchymal stem cells

conditioned media enhances cooled sperm parameters

Feriel Yasmine Mahiddine¹, Min Jung Kim¹

¹Department of Theriogenology and biotechnologies, Seoul National University, Seoul 08826, South Korea

Sperm chilling is a widely used biotechnology in dog breeding facilities for shipping and artificial insemination.
When used for short storage time, it delivers better results than frozen sperm but kinetic parameters could be
enhanced by the addition of anti-oxidants to reduce reactive oxygen species production. Here, we evaluated the

effects of conditioned media (CM) from canine amniotic membrane mesenchymal stem cells in the chilling extender.

Sperm was cooled for 4h using chilling media with concentrations of 0, 5, 10, 15% of CM respectively. After washing,
kinetic parameters were then evaluated using CASA (FSA2011 premium edition version 2011), viability and
membrane integrity were evaluated with eosin-nigrosin and hypo osmotic swelling test respectively. Statistical

analysis was performed using GraphPad Prism 5.0. Addition of 10% and 15% CM significantly enhanced sperm
kinetic parameters compared to control group, with 10% CM group showing higher motility results; however, they

showed no significant differences in sperm viability and membrane integrity. We can conclude that addition of 10%
CM in the extender improves sperm chilling. This study was supported by RDA (CCAR, #PJ014786012020), Research

Institute for Veterinary Science.