Page 12 - A. Aging
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[A. Aging-7]
Choline Acetyltransferase induces the functional regeneration
of salivary gland in aging mouse model
TOAN KHANH NGUYEN¹, TUONG MANH NGUYEN¹, SEHO JEONG¹, SANG-GUN AHN¹
¹Pathology, School of Dentistry, Chosun University, Gwangju 61452, Republic of Korea
This study aimed to investigate the impact of aging in the salivary gland by metabolomics approach in the extensive
aging mice model, SAMP1/Klotho -/- mice. We found that the salivary secretion of SAMP1/Klotho -/- mice
decreased dramatically, compare with SAMP1/Klotho WT. Metabolomics profiling analysis showed that the level of
Acetylcholine significantly decreased in SAMP1/Klotho (-/-) mice, although the corresponding level of Acetylcholine
precursors, Acetyl-CoA and Choline, increased. Interestingly, the mRNA and protein expression of Choline
Acetyltransferase (ChAT), which is responsible for catalyze the Acetylcholine synthesis, importantly decreased in
SAMP1/Klotho -/- mice. The overexpression of ChAT induced the expression of salivary gland functional markers (α
– amylase, ZO-1, and Aqua5) in primary cultured salivary gland cells from SAMP1/Klotho +/+ and -/- mice. In in
vivo study, AAV-ChAT transduction significantly increased saliva secretion compared with the control in
SAMP1/Klotho -/- mice. These results suggest that the dysfunction in biosynthesis of Acetylcholine by ChAT
reduction may cause the impairment in salivary gland function.
(This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea
government (MSIT) (No. 2018001260
