Page 7 - A. Aging
P. 7
A 2.0 B C
Relative Cellular Proliferation
AKT inhibitors 125 125
**
1.5 100 100
Lysosomal Mass (Relative to DMSO) 50 Autofluorescence (Relative to DMSO)
1.0 75 ** 75 **
0.5 25 50
25
0.0 0 0
GDC-0068 AZD5363 GSK690693 PF-04691502 TIC10 Triciribine Honokiol PHT-427 AT7867 A-674563 MK-2206 2HCl CCT128930 DMSO GDC0068 DMSO GDC0068
A B C D
1 2 5 * 125 125 Rotenone+
1 0 0
100
100
Senescent (GDC0068)
80
Oligomycin
MMP (Relative to GDC0068) 7 5 ROS (DHR123) (Relative to DMSO) 75 * ROS (MitoSOX) (Relative to DMSO) 75 * OCR(pmol/min) 100 Senescent (DMSO) FCCP Antimycin
60
5 0
50
50
40
2 5
0 25 25 20
0
0
0
DMSO GDC0068 DMSO GDC0068 DMSO GDC0068 -20 10 20 30 40 Time(min) 60 70 80 90 100
50
with Chloroquine
A LC3B Mitochondria Merged B C D
10 μm LC3B Mitochondria Merged
YOUNG 1.2 ** YOUNG 100
10μm 10μm 10μm 10μm 0.8 10μm 10μm 10μm 10μm 80
DMSO CYTO-ID Green DMSO Mitochodrial Mass 60 **
SENESCENT 10μm 10μm 10μm 10μm 0.4 SENESCENT 10μm 10μm 10μm 10μm 20
40
GDC0068 0.0 GDC0068 0
10μm 10μm 10μm 10μm DMSO GDC0068 10 μm 10μm 10μm 10μm DMSO GDC0068
10μm
