Page 248 - ebook

[D. Medicine/Translational Research] D-8

Elevated miR-93-5p from extracted thrombus is associated

with recurrent vascular event after cerebral infarction

Jeong-Min Kim¹*, Sun-Uk Hong², Sungguan Hong³, Kwang-Yeol Park⁴, Hye Ryoun Kim⁵

¹Department of Neurology, Seoul National University Hospital, Seoul 03080, Korea, ²Department of Pathology,

Chung-Ang University Hospital, Chung-Ang University College of Medicine, Seoul 06973, Korea, ³Department of

Chemistry, Chung-Ang University, Seoul 06974, Korea, ⁴Department of Neurology, Chung-Ang University Hospital,
Chung-Ang University College of Medicine, Seoul 06974, Korea, ⁵Department of Laboratory Medicine, Chung-Ang
University Hospital, Chung-Ang University College of Medicine, Seoul 06974, Korea

Background: We investigated whether microRNA expression pattern can provide information about neurological
deterioration among acute ischemic stroke patients. Methods: Between 2015 and 2020, acute ischemic stroke

patients who had been through mechanical thrombectomy from Chung-Ang University Hospital were included.
After informed consent we harvested thrombus at -70℃. The expression signature of microRNAs was evaluated by

microarray analysis. The significantly altered microRNAs were validated by quantitative real time polymerase chain
reaction. Correlation analysis was performed to study relationship between significantly altered microRNAs and

laboratory variables. Results: Among 95 patients who received mechanical thrombectomy, a total of 78 patients
were finally included in the study after excluding 9 patients without thrombus and 8 patients without undetectable

microRNAs. Recurrent vascular event was detected in 13 patients. Microarray analysis comparing 1088 microRNAs
between the patients with and without recurrent vascular event and validation by RT-PCR showed that miR-93-5p
and miR-20a-5p were significantly elevated among the patients with RVE. Both microRNAs were significantly

elevated among the patients with poor prognosis and positively correlated with discharge NIHSS. Correlation

analysis found that the expression levels of miR-93-5p was positively associated with E/e’ ratio and serum
parathyroid hormone. The expression of miR-20-5p was positively associated with platelet count,