Page 72 - ebook

[B. Cell Biology/Stem Cell] B-36

Umbilical Cord Blood Ameliorated Neurological Dysfunction

and Mitochondrial Dynamics in Collagenase induced

Intracerebral Hemorrhage rat model

Sung Chan Ha¹, Jin Seung Choung¹, Jee In Choi¹,², Joo-Wan Choi¹, Min Young Kim¹,²*

¹Department of Rehabilitation Medicine, CHA Bundang Medical Center, CHA University School of Medicine,

Seongnam 13496, Korea, ²Rehabilitation and Regeneration Research Center, CHA University School of Medicine,

Seongnam 13488, Korea

Intracerebral hemorrhage (ICH) has the highest mortality rate of all stroke subtypes with limited therapeutic options.
Human umbilical cord blood (hUCB) cell therapy is a promising treatment of stroke, however the molecular

mechanisms and target markers of therapeutic effect are still elusive. Mitochondria play an important role in cell
growth and neuronal survival including fission and fusion of mitochondria, which are thought to be crucial for

recovering dysfunction in stroke. The aim of this study was to elucidate the mechanisms of mitochondrial protection
involved in mitochondrial dynamics with hUCB treatment for ICH. Our results showed that hUCB treatment increased

mRNA expressions of fusion molecules, mitofusion-2 (MFN-2) and optic atrophy-1 (OPA-1) compared to the ICH.
Decreased mRNA levels of mitochondrial fission marker, dynamin-1-like protein (DRP-1) and mitochondrial fission-
1 (FIS-1) were restored after hUCB treated. For the anti-apoptotic effect in protein level, bcl-2-like protein 4 (BAX)

/ B-cell lymphoma 2 (BCL-2) ratio was declined. Also, the hUCB improves neurological deficits via modified

neurological severity (mNSS) score. In conclusion, this study suggests that hUCB treatment improves mitochondrial
dynamics and ameliorates anti-apoptotic effects and motor dysfunction.