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Study on the mechanisms of m A modification regulating Histone mRNA expression
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Hwang Jun seok 1,2,3 , Kim min kyung 1,2,3 , Seung Hun Han , Jang seongmin , Junho Choe 1,2,*
1,2
1,2
1 Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, Korea
2 Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Korea
3 These authors contributed equally to this work
Research objective Background of the study

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• Study of gene expression and molecular mechanism by m A
modification of Histone mRNA.
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• Study of a Reader protein attached to m A -modified Histone mRNA
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• Study on sequence-specific Histone mRNA m A modification position
and expression regulation
Research content

[Histone mRNA processing, stability, and translation verification [Figure 1] Schematic diagram showing the difference between Histone mRNA
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through m A modification] and general mRNA.
- After down-regulation Methyltransferase(MT) and
Demethyltransferase(DMT), Histone mRNA and pre-mRNA are analyzed
to verify their effect on Histone mRNA stability.
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- Check the effect of m A on the stability of Histone mRNA. By measuring
the amount of Histone mRNA degraded by treating Hydroxyurea (HU)
- After synchronizing the cell cycle, changes in Histone mRNA and pre-
mRNA according to m A regulation were analyzed to confirm the effect
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of m A on the processing and stability of mRNA.
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Research results and expected effects [Figure 2] Control of the expression of [Figure 3] m 6 A regulation mRNA
metabolic regulation diagram.
Histone mRNA according to the cell cycle.
Histone-1 mRNA Histone-2 mRNA
• Research Results
[Figure 4] Western blot results
The knock down efficiency of siRNA was confirmed as a Western blot.
It was confirmed that both MT gene and DMT gene were knocked down
by about 1/9.

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[Figure 5] m A dependent Histone mRNA processing & degradation
After synchronizing the cell cycle, confirmation of changes in Histone
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[Figure 4] western blot results [Figure 6] m A dependent Histone mRNA stability mRNA according to m A modification. In the case of MT gene, it can be
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confirmed that the amount of Histone mRNA increases as down-
Histone-1 mRNA Histone-2 mRNA
regulation, but in the case of DMT gene, it decreases.
[Figure 6] m A dependent Histone mRNA stability
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In the case of MT gene, it can be seen that both Histone-1-mRNA and
Histone-2-mRNA increased after HU treat. However, in the case of B
gene, it was confirmed that all of them decreased.
• Expected effects
- Inhibitors of reader proteins that specifically bind to Histone mRNA
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m A modification will be discovered and developed.
- By revealing the mechanism of regulating Histone mRNA expression
through RNA modification, Development of new concept treatments
and inhibitors for genetic diseases and cancer cells caused by cell
division problems.
- Provide an opportunity to introduce ‘Epitranscriptomics’ to domestic
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[Figure 5] m A dependent Histone mRNA processing & degradation researchers and students.
Further study

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• Measurement of the efficiency of Histone mRNA translation according to m A mRNA modification through a Polyosome profiling experiment.
• Check the cell cycle when down regulating methylated enzymes or demethylated enzymes by FACS(Fluorescence-activated cell sorting).