Page 110 - ebook

[A. Biochemistry/Molecular Biology] A-55

Natural antioxidants from Schizandra chinensis stem enhance

anti-inflammatory activities with modulations of Nf-kB-

regulalted pro-inflammatory cytokines in LPS-induced Raw

264.7 cells

Yeo Won Jeoung¹, Daeun Kim², Yunok Oh³*

¹Department of Cosmeceutical Science, Daegu Haany University, Daegu 38578, Korea, ²Department of Cosmetic

Science, Kyungsung University, Busan 48434, Korea, ³Convergence Research Center for SmartHealthcare,
Kyungsung University, Busan 48434, Korea

Schizandra chinensis seed is widely used as a popular food, spice, and traditional medicine, which contains several
bioactive substances with anti-inflammatory and antioxidant properties. Recently, Schizandra chinensis stem (SCS)

has been recently found to have anti-oxidative and anti-inflammatory activities, but the therapeutic effects of SCS
have not yet been sufficiently investigated. In this study, the main phenolic compounds as antioxidants from SCS

were extracted using 70% ethanol and hot water. Phenolic contents in SCS were analyzed by UV-vis spectroscopy
and HPLC method. SCS showed good antioxidant activity on DPPPH assay in a dose-dependent manner. SCS also

showed good anti-inflammatory activities through suppression of Nf-kB-mediated pro-inflammatory cytokines, such
as TNF-α, IL-16, and IL-1β. Western blot analysis conducted downregulation of iNOS and COX-2 protein expression
in LPS-induced Raw 264.7 cells. Taken together, these results suggest that SCS could be used as functional food

ingredients for effective anti-oxidative and anti-inflammatory activities.