Page 2 - X. Stem cell biology

[X. Stem cell biology-1]

Exosomes derived from feline adipose tissue mesenchymal

stem cells reduce inflammation factors

Soo-Eun Sung¹, Kyung-Ku Kang¹, Joo-Hee Choi¹, Si-Joon Lee¹, Minkyoung Sung¹, Kil-Soo Kim¹, Min-Soo Seo¹˙*

¹Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 41061, Republic of Korea

Adipose tissue derived mesenchymal stem cells (AD-MSCs) release extracellular vesicles such as exosomes and
microparticles. Particularly, exosomes are formed inward a cell, throughout multivesicular bodies (MVB), so the
contents protein, DNA, and RNA are similar to the parent cells. The exosomes research is rapidly expanding, many

studies published in recent years, but the function and application of MSC-derived exosomes should emerge as

cell-free therapeutics compared with well-known mesenchymal stem cells therapeutic effects. We isolated exosomes
using ultracentrifugation from feline AD-MSCs and feline fibroblasts cell culture medium. Characterization of feline
exosomes using FACS, DLS, NTA and TEM imaging. The exosomes had typical size about 100 nm and expressed

tetraspanins such as CD9, CD81. And then, we analyzed cytokines and chemokines levels from exosomes by ELISA.
As a result, anti-inflammatory factor IL-10 increased in AD-MSCs exosomes, pro-inflammatory factors such as IL-1

beta, IL-8 and IFN-gamma decreased in AD-MSC exosomes. Our work is the first demonstrated that feline AD-
MSCs derived exosomes enhanced the inflammatory suppressive effects and it had the therapeutic potential of

immune disease.