Page 45 - N. Metabolism and metabolic diseases

Ginsenoside Compound K regulates adipogenesis
in vitro and in vivo

Sun Young Park 1,2 and Sungkun Chun 1,2
2
1 Department of Physiology, Brain Korea 21 plus Program, Jeonbuk National University Medical School,
Jeonju-si, Jeollabuk-do 54907, Korea
Abstract
Panax ginseng has been used as a natural medicine and their main component ginsenosides were reported that have many therapeutic effects
for cancer, angiogenic disease, diabetes, obesity and neurological diseases. Recently, several studies showed that some types of ginsenosides
have anti-obesity effects. However, ginsenoside Compound K’s effect on obesity or lipid metabolism was not reported yet. In this study, the effects
of compound K on lipid metabolism were investigated through in vivo and in vitro assays. 3T3-L1 cells were differentiated with or without CK
treatment and the related genes or proteins were examined. In addition, white adipose tissue (WAT) and brown adipose tissue (BAT) from
C57BL/6 mice was used to confirm CK’s regulating effects on adipogenesis of both tissues. As a result, CK treatment regulated the differentiation
of 3T3-L1 cells via concentration-dependent manner. Intraperitoneal CK injection (10mg/Kg, 10 days) increased expression of UCP-1 and PGC1-
α mRNA levels in WAT as well as reduced the size of lipid droplets in WAT of CK-treated groups (n=5) compared to the vehicle-treated groups
(n=5). In conclusion, these data suggest ginsenoside CK treatment can induce transition from white adipocytes to Brite (beige) adipocytes and
have potential roles for treatment of obesity or lipid metabolic disorders.
Methods Experimental scheme

In vitro

In vivo

Sacrifice

Results
In vitro assays In vivo assays
1. CK reduced the body weight and WAT mass
1. Effects of CK on viability of 3T3-L1 preadipocytes
A B

2. CK promoted the expression of thermogenesis marker genes in C57BL/6
2. Effects of CK on the lipogenesis and lipid accumulation of 3T3-L1 mice
cells
A

3. CK reduced the adipocyte size in WAT
B C

4. IF results show that CK treatment group has more high UCP1 expression
than CTL and CTL has big lipid than CK treatment group in Oil red O staining.
3. CK promoted the expression of thermogenesis marker genes in
mature 3T3-L1 adipocytes

Conclusion REFERENCES
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therapeutic approach for obesity or metabolic disorders. This work was supported from the Korea Health Industry Development Institute (KHIDI, HI17C1510)
and the National Research Foundation(NRF-2019M3C7A1032551).