Page 2 - M. Immunology

[M. Immunology-2]

Tat-thioredoxin1 exert anti-inflammation effects by reduction

of MAPK and NF-κB activation

, Yeon Joo Choi¹, Hyeon Ji Yeo¹, Dae Won Kim², Eun Jeong Sohn¹, Hyun Ju Cha¹, Jinseu Park¹, Kyu
Hyung Han¹, Sung Ho Lee³, Sunghou Lee⁴, Duk-Soo Kim⁵, Won Sik Eum¹, Min Jea Shin¹, Eun Ji Yeo¹,

Soo Young Choi¹

¹Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University,
Chuncheon 24252, Korea, ²Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences,
College of De, Gangneung-Wonju National University, Gangneung 25457, Korea, ³Genesen Inc., Teheran-ro,
Gangnam-gu, Seoul 06181, Korea, ⁴Department of Green Chemical Engineering, Sangmyung University, Cheonan
31066, Korea, ⁵Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan-Si 31538,

Korea

Inflammation triggers many interconnected pathophysiological events including neuronal diseases and several anti-
inflammatory and anti-oxidant proteins have protective effects on neuronal cell damage. Since thioredoxin 1 (Trx1)
protein is known to reduce cell death by regulation of anti-oxidant and anti-apoptotic functions, we explored

whether cell permeable Tat-Trx1 protein showed beneficial influences inflammatory response in lipopolysaccharide
(LPS)-induced Raw 264.7 cells and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced animal model. Transduced

Tat-Trx1 protein significantly reduced cell damages by regulation of MAPK and NF-κB activation in LPS-treated cells
and this fusion protein modulated apoptosis signaling pathway and inflammatory responses. Furthermore, Tat-Trx1

protein transduced into skin tissues and markedly reduced inflammatory responses. These findings indicate that
transduced Tat-Trx1 protein paly a beneficial roles in the inflammation, suggesting it may be a potential therapeutic

protein drug for inflammatory disorders including skin and dry eye.