Page 19 - L. Genetics and genomics

Genetic variation in mitochondrial NADH-dehydrogenase subunit 3 (ND3) is
associated with susceptibility to gastric cancer
Gang Min Hur , Jang Hee Hong 1,2
1
2
1 Department of Pharmacology, Chungnam National University College of Medicine, Daejeon, Republic of Korea, Clinical
Trials Center, Chungnam National University Hospital, Daejeon, Republic of Korea.
BACKGROUND & AIM

Several studies have been reported that mitochondrial DNA alterations are related with cancer development and progression. In
this study, we performed case-control association studies to evaluate the relevance between mitochondrial NADH-
dehydrogenase subunit 3 (ND3) gene polymorphisms and the risk of gastric cancer (GC) in the Korean population.

METHODS

A case-control study was conducted with 377 GC patients and 363 controls. We genotyped 6 SNPs such as rs28358278,
2
rs2853826, rs201397417, rs41467651, and rs28358275 in ND3 gene using direct sequencing. χ tests were used to estimate
the Hardy-Weinberg equilibrium of each SNP and the association between the GC and control. Binary logistic regression was
conducted to estimate the GC risk by odds ratios (OR) and 95% confidence intervals (CI).

RESULTS

Variant, rs41467651 was significantly associated with an increased gastric cancer risk of GC and diffuse-type GC (OR = 2.08, P
= 0.006 and OR = 2.64, P = 0.002, respectively).

Table 1. Genotype and allele frequencies of ND3 Table 2. Stratified analysis of ND3 polymorphisms in GC
polymorphisms among GC patients and controls patients and controls by gender

SNP, single nucleotide polymorphism; GC, gastric cancer; CON, controls; OR, odds ratio; SNP, single nucleotide polymorphism; GC, gastric cancer; CON, controls; OR, odds ratio; CI,
CI, confidence interval. a Adjusted for gender and age. confidence interval. a Adjusted for gender and age.

Table 3. Stratified analysis of ND3 polymorphisms in GC patients and controls by gender

SNP, single nucleotide polymorphism; GC, gastric cancer; CON, controls; OR, odds ratio; CI, confidence interval. a Adjusted for gender and age.
CONCLUSION

Our data suggests that rs41467651 SNP of ND3 gene may influence ND3-mediated signaling pathway, particularly in the
development of diffuse-type GC in the Korean population.
Contact information

Jang Hee Hong: boniii@cnu.ac.kr