Page 21 - L. Genetics and genomics

Association of Genetic variants in long non-coding RNA SOX2OT with risk of
gastric cancer
Gang Min Hur , Jang Hee Hong 1,2
1
2
1 Department of Pharmacology, Chungnam National University College of Medicine, Daejeon, Republic of Korea, Clinical
Trials Center, Chungnam National University Hospital, Daejeon, Republic of Korea.
BACKGROUND & AIM

Long non-coding RNAs (LncRNAs) have been suggested that are associated with cancer progression. In this study, we
evaluated the association between polymorphism rs9839776 in lncRNA sex-determining region Y-box 2 overlapping transcript
(SOX2OT) and the risk of gastric cancer (GC) and GC subgroups via a case-control study.

METHODS

We genotyped polymorphism rs9839776 in SOX2OT with 377 GC patients and 353 controls using TaqMan genotyping assay. χ 2
tests were used to estimate the Hardy-Weinberg equilibrium of each SNP and the association between the GC and control.
Binary logistic regression was conducted to estimate the GC risk by odds ratios (OR) and 95% confidence intervals (CI).
RESULTS

The rs9839776 CT/TT genotype and T allele showed significant association with a decreased risk of GC (CT/TT: adjusted OR =
0.72, 95% CI = 0.52-1.00, P = 0.046; T: adjusted OR = 0.74, 95% CI = 0.56-0.99, P = 0.044). Stratified analysis also revealed
that CT and CT/TT genotypes, and T allele of rs9839776 was significantly associated with a decreased risk of GC showing LNM
negative (CT: adjusted OR = 0.67, 95% CI = 0.45-0.99, P = 0.044; CT/TT: adjusted OR = 0.67, 95% CI = 0.46-0.98, P = 0.039;
T: adjusted OR = 0.71, 95% CI = 0.51-1.00, P = 0.0491) and GC in tumor stage I+II (CT: adjusted OR = 0.65, 95% CI = 0.45-
0.95, P = 0.028; CT/TT: adjusted OR = 0.66, 95% CI = 0.50-0.96, P = 0.028; T: adjusted OR = 0.71, 95% CI = 0.52-0.99, P =
0.041).
Table 1. Genotype and allele frequencies of SOX2OT polymorphisms among GC patients and controls

a
GC, gastric cancer; CON, controls; OR, odds ratio; CI, confidence interval. Adjusted for gender and age.
Table 2. Stratified analysis of ND3 polymorphisms in GC patients and controls by gender

a
GC, gastric cancer; CON, controls; OR, odds ratio; CI, confidence interval. Adjusted for gender and age.
CONCLUSION

Our data suggests that SNPs of lncRNA SOX2OT may be a useful biomarker for gastric cancer. However, further functional
studies and researches in other ethnic groups with larger sample size are required to confirm our findings.
Contact information

Jang Hee Hong: boniii@cnu.ac.kr