Page 30 - D. Cancer biology

[D. Cancer biology-23]

CD133-containing microvesicles transport SNAI1 and

promote cell motility in colon cancer

Jin Suk Pyo¹, Jesang Ko¹

¹Life Sciences, Korea University Graduate School, Seoul 02841, Republic of Korea

Extracellular vesicles (EV) derived from tumor microenvironment carry numerous bioactive molecules to deliver to
recipient cells. Microvesicles (MV) are a type of EV that are released from the cell membrane and play an important
role in cell-to-cell communication. Epithelial-to-mesenchymal transition (EMT) is considered as a pivotal procedure

of carcinogenesis, cancer cell mobility, and metastasis. However, it is not yet clear whether EMT-related molecules

are delivered by MVs. We reported that CD133 modulates MV release and oncoprotein trafficking in colon cancer.
In this study, we investigated the role of CD133-containing MVs in the transport of EMT-related molecules in colon
cancer. We examined the components of MVs derived from CD133-positive and -knockdown HCT116 colon cancer

cells. Among various EMT-related proteins, only SNAI1 was carried by MVs from CD133-positive cells and
transported to recipient cells. The delivered SNAI1 up-regulated the expression of ZEB1 and Vimentin in recipient

cells. In addition, CD133-containing MVs promoted migration and invasion of recipient cells. However, MVs from
CD133-knockdown cells did not affect the motility of recipient cells. These results indicate that CD133-containing

MVs are involved in regulating cell motility by delivering SNAI1