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1. Introduction 3. Results
Implantable medical devices are used for support of regenerating and replacing Characterization of microsphere
organs and tissues in an incapable state. However, many people who use medical
devices suffer various complications, such as medical device-related infections. The
current approaches to prevent and treat these infections include disinfection of
medical devices before their implantation and multiple-dose systemic
administration of antibiotics and. However, these solutions often result in a low
effectiveness, short-term effect and chance of bacterial resistance. Importantly,
bacteria can form biofilms on medical devices, which is effectively resistant to
antibiotics. In this study, we aimed to devise a method of bio-inspiredly modifying
the silicone surface with antibiotic microsphere depots and multi-layered Figure 6. The SEM images depicted discrete microspheres with the size of 0.5-7 μm,
polyelectrolytes for long-term prevention of biofilm deposition. Poly(lactic-co- which was consistent with the dynamic size. Average of VAN-PDMS size is 2.84±1.42
glycolic acid) microspheres (MS, negative charge) were used as biocompatible . In the FT-IR spectrum, the spectra of Cou-6 MS showed no newly formed peaks
depots for sustained release of antibiotic drug, whereas multi-layered compared with that of Cou-6 and Blank MS.
polyelectrolytes composed of quaternized chitosan (HTCC, positive charge) and Conjugation of Microsphere on Silicone surface
polyacrylic acid (PAA, negative charge) were used for achieving a contact-killing
effect.

2
Figure 7. The maximum conjugated microsphere amount is about 5 μg/mm . In
addition, the optimal condition is incubation of silicone surface in 4 mg/mL
Figure 1. Study Design microsphere suspension for 15 minutes.
2. Methodology
1) Synthesis of HTCC Polyelectrolyte Multilayer Coating on Silicone Surfaces
a b

(GTMAC)
HClO 4

Figure 2. Synthesis of HTCC
12 µm 60µm
2) Fabrication of microsphere c d e

15 µm
12 µm

Figure 3. Fabrication of microsphere
3) Modification and Conjugation of microsphere on silicone surfaces
Figure 8. Characterization of PEM coating on silicone surface. (a) SEM image without
PEM coating, (b) SEM image with PEM coating, (c) Thickness of PEM coating layer,
(d) Loss of microsphere in coating process, (e) Water contact angle of PEM coating

4. Conclusion
Through this study, to prevent bacterial infection that may occur during
medical devices, we established a novel surface modification method of the
silicone surface through biocompatible hydrogel and sustained-release PLGA
microsphere. The morphology and various characteristics of this coating
system were demonstrated.In the future, it is necessary to demonstrate the
Figure 4. Modification surface and Conjugation of microsphere on silicone surface antibacterial effect on the PEM coated surface with antibiotics loaded PLGA
4) Polyelectrolyte Multilayer (PEM) coating on silicone surface microsphere.
References
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Ghlam Nabi, Fordyce Davidson, Geoffrey Michael Gadd, and Qi Zhao. Enhanced
Antibacterial and Antiadhesive Activities of Silver-PTFE Nanocomposite Coating for
Urinary Catheters. ACS Biomaterials Science & Engineering 2019,5, 2804-2814.
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bearing interface. Wiley society for biomaterials
4. Hirenkumar K.Makadia, and Steven J.siegel. Poly Lactic-co-Glycolic Acid (PLGA) as
Figure 5. Polyelectrolyte Multilayer (PEM) coating on silicone surface Biodegradable Controlled Drug Delivery Carrier. Polymers 2011,3,1377-1397