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Novel Bevemovirus and Celavirus Identified in
the Striga hermonthica RNA-Seq Data

Dongjin Choi, Chaerim Shin, and Yoonsoo Hahn
Department of Life Science, Chung-Ang University, Seoul 06974, South Korea
Identification of novel potyvirid viruses

 RNA-Seq data obtained from plant tissues often contain viral sequences
derived from latently infected RNA viruses.
 Potyvirids (family Potyviridae) are the largest family of plant RNA viruses.

 Two novel potyvirid viruses, Striga-associated poty-like virus 1 (SaPlV1)
and Striga-associated poty-like virus 2 (SaPlV2), were identified from the
RNA-Seq data of purple witchweed (Striga hermonthica).

SaPlV1 genome

 The SaPlV1 genome encodes a 2462-amino acid (aa)  The cleavage sites of SaPlV1, BVMoV, and macluravirus
polyprotein that may be cleaved into nine mature polyproteins shared strong sequence similarities.
peptides.

SaPlV2 genome
 The SaPlV2 polyprotein contained 3329 aa; it may be cleaved into at least seven or eight mature peptides.

Phylogenetic positions of SaPlV1 and SaPlV2

 SaPlV1 was most closely related to bellflower vein mottle virus
(BVMoV), the only member of the genus Bevemovirus, and then to
macluraviruses (genus Macluravirus).
 SaPlV2 was most closely related to celery latent virus, the sole species
of the genus Celavirus, which is the most divergent potyvirid genus.
 Phylogenetic analysis suggested that SaPlV1 and SaPlV2 may be novel
species of the genera Bevemovirus and Celavirus, respectively.
 The genome sequences of SaPlV1 and SaPlV2 are useful resources for
studying the genome evolution of potyvirids.

 This research was published in Acta Virologica (2021).
 This research was supported by grants from the National Research Foundation
of Korea funded by the Government of Korea (grant Nos. 2018R1A5A1025077
and 2020R1A2C1013403)