Page 168 - ebook

[B. Cell Biology/Stem Cell] B-19

Differentiation of Various Gastric Cancer Cell Lines using

Glycome Signatures by LC-MS/MS

Jihyeon Nam¹,², Nari Seo¹,², J Eugene Lee³, Hyun Joo An¹,²*

¹Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 34134, Korea,

²Asia-Pacific Glycomics Reference Site, Chungnam National University, Daejeon 34134, Korea, ³Center for

Bioanalysis, Korea Research Institute of Standards and Science, Daejeon 34113, Korea

Glycosylation, which is highly sensitive to the external environment, has been considered as a window reflecting
physiological state of cell. Since abnormal glycans are a hallmark of diseases, the characterization of glycans is

gaining attention in biomarker research for treatment and diagnosis. In particular, gastric cancer (GC) with various
subtypes has highly different clinical results for each patient. Although gastric cancer patients are classified into

different subgroups according to genetic profiling and clinical symptoms, targeted treatment is difficult. In this study,
we have characterized the glycans obtained from representative GC cell. In order to selectively separate the glycans

located on the cell surface, the cell membrane extraction was first performed. Subsequently, glycans were
enzymatically released, enriched, and profiled via PGC nanoLC/MS and LC/MS/MS. Using normalized glycan intensity,

a correlation among GC cell lines was examined through hierarchical analysis. The heterogeneity of each cell line
was distinguished by moietise of glycans such as fucosylation, sialylation, LacdiNAc and bisecting structures. Our

findings on GC cell line-specific glycans may pave the way for accurate diagnosis of heterogeneous gastric cancer
and the potential for molecular target recognition for gastric cancer treatment.