Page 41 - ebook

[F. Others] F-20

Design of CNT-based electron microbeam cell irradiation

system for cell biology research on low-dose radiation

Hanna Lee¹, Jinho Choi², Amar Prasad Gupta³,⁴, Jaeik Jung⁴, Jaekyu Jang⁴, Kyung-Sik Yoon⁵*,

Jehwang Ryu⁶*

¹Department of Age Service-Tech Convergence, College of Medicine, Kyung Hee University, Seoul 02447, Korea,

²Department of Radiation Oncology, College of Medicine, Kyung Hee University, Seoul 02447, Korea, ³Department
of Physics, Kyung Hee University, Seoul 02447, Korea, ⁴R&D, CAT Beam Tech Co. Ltd, Seoul 02455, Korea, ⁵

Department of Biochemistry and Molecular Biology, College of Medicine, Kyung Hee University, Seoul 02447,
Korea

1. Description of purpose Radiation research is mostly aimed at improving the use of radiation such as radiation

therapy and materials. Although the effect of high-dose radiation in cells has been studied a lot, the effect of low-
dose radiation still needs a lot of scientific evidence. Therefore, we study the cellular response to low-dose radiation

from the development of CNT cold electron cathode microbeam cell irradiator. 2. Methods A. Microbeam Cell
Irradiation System The microbeam source was developed as a CNT-based field emission source. CNTs were grown

by direct chemical vapor deposition on a metal alloy substrate using catalysts such as Fe and Ni, and field emission
characteristics were confirmed. B. Cell Irradiation The effect of microbeam irradiation on cells was confirmed by

examining DNA, cytoskeleton, and number of cells. 3. Conclusion We were able to set the in-vitro conditions of the
developed microbeam system. In addition, by confirming the effect of cells on low-dose radiation, it was possible

to understand the microscopic phenomena of cells exposed to low-dose radiation. By improving the precision of
the system, it will be possible to expand its application to lesion cells with low doses in the future.