Page 23 - ebook

[F. Others] F-11

Repeated dose 28-day oral toxicity study of Platycodon

grandiflorum mixed herb extracts in mice

Jin Won Park¹, Do-Hyung Kim¹, Sungjin Lee¹, Hae Li Ko¹, Deuk-ki Lee¹, Younghyeon Kim², Sol Lee³,

Deok-Hoon Kong¹, Sang-In Park¹*

¹Division of Research Program, Scripps Korea Antibody Institute, Chuncheon 24341, Korea, ²Department of

Systems Immunology, Kangwon National University, Chuncheon 24341, Korea, ³Geoduri Korean Medicine Clinic,
Geoduri Korean Medicine Clinic, Chuncheon 244, Korea

The objective of this study was to obtain repeated oral dose 28-day toxicity information of Platycodon grandiflorum

mixed herb extracts(PMEs) in female and male ICR mice. In order to investigate the toxicity and identify target
organs, test articles were orally administered to female and male ICR mice, once-in-a-lifetime 28 days an

experimental period, at the dose of levels of 2,000, 1,000, 500 and 0 (vehicle control) mg/kg (of body weights), in
a volume of 10 ml/kg (dissolved in distilled water, as vehicle). The highest dosage used in the present study was

selected as 2,000 mg/kg in consideration of single dose oral toxicity study, and 1.000 and 500 mg/kg were selected
as middle and lower dosage groups using common ratio 2, respectively. As results of repeated dose oral treatment

of PMEs, there were no changes related to treatment in mortality, clinical signs, body weights and gains, organ
weights, hematological inspections and gross necropsy, up to 2,000 mg/kg, as compared with those of equal genders

of vehicle control mice. The results obtains in this study suggest that PMEs in non-toxic in mice up to 2,000 mg/kg,
and is therefore likely to be safe for a health functional