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[F. Others] F-6

Screening of plants for treating osteoporosis based on ALP

activity from Endemic Species of Korea

Soyeon Hong¹,², Da Seul Jung¹, Jae-Eok Park¹, Jung-Hye Choi², Chu Won Nho¹*, Gyhye Yoo¹*

¹Smart Farm Research Center, Gangneung Institute of Natural Products Korea Institute of Science and Technology

(KIST), Gangneung, Gangwon-do 25451, Korea, ²KHU-KIST Department of Converging Science and Technology,

Kyung Hee University, Seoul 130-701, Korea

Osteoporosis is a bone disorder with low bone density and its incidence ranges from one in 2 women after 50.
Osteoporosis is caused by an imbalance in the bone remodeling between bone formation and destruction. In the

present study, we screened endemic Species of Korea having osteogenic activity to find a cure for osteoporosis.
ALP (alkaline phosphatase) activity in MC3T3-E1 preosteoblasts was used to estimate osteogenic activity for

screening. Ethanol extracts of 20 species of plants and their fractions were prepared and utilized for screening.
During 6 days of osteoblast differentiation of MC3T3-E1 cells, extracts or fractions were treated and ALP activity

were measured. Among 20 species of plant extracts, three extracts, BPRL and BPAP elevated ALP activity significantly.
Among fractions of 2 selected plants, their hexane fractions were most effective on ALP activation. Those activities

were confirmed by Western blot: BPRL-Hex elevated the level of RUNX2, Osterix and type 1 collagen alpha 1
(Col1a1). BPAP-Hex and elevated the level of RUNX2 and Col1a1. In conclusion, we found 2 novel endemic species

in Korea for osteoporosis, and plan to conduct animal experiments using ovariectomized mice and find active
compounds from BPRL-Hex-F3.