Page 62 - ebook

[B. Cell Biology/Stem Cell] B-31

Chromatin Remodeling Factor RSF1, regulate p53

transcription activation through multivalent interaction

Heo Yungyeong¹ , Min Sunwoo³,⁴ , Kim Yonghyeon¹, Choi Yong Won², Cho Hyeseong³,⁴*
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¹Biomedical Sciences, Ajou University School of Medicine, Suwon 16499, Korea, ²Hematology-Oncology,, Suwon,

Korea, Ajou University School of Medicine, Suwon 16499, Korea, ³Biochemistry, Ajou University School of

Medicine, Suwon 16499, Korea, ⁴Genomic Instability Research Center, Ajou University School of Medicine, Suwon
16499, Korea

As a member of the imitation switch (ISWI) family in ATP-dependent chromatin remodeling factor, remodeling and

spacing factor (RSF) complex consisting of two subunits, SNF2h ATPase and RSF1. Chromatin-remodeling complexes
are important for an appropriate DNA damage response and repair and essential for maintaining genomic stability.

Activation of p53 occurs by several mechanisms including protein stabilization and modification of a protein by
acetylation. RSF1 had been initially found in the complex with FACT (facilitates chromatin transcription) to reposition

the nucleosome for transcriptional regulation. Recent studies revealed that RSF1 controls p53-mediated transcription
in apoptosis upon DNA strand breaks and reduced transcription of p53-target genes as a potential mechanism.

However, the mechanism of p53 transcription in RSF1 KO cells after DNA damage is not fully understood yet. We
showed that our data, RSF1 KO reduced p53 acetylation level, the fold enrichment of acetyltransferase p300 at

p53RE sites, but increase fold enrichment of deacetylase HDAC1. Also find that RSF1 is an important factor that
enables transcription to be maintained by regulating the enhancer marker and PIC complex. Taken together, our
data indicate that RSF1 contributes to transcriptional regulation and gene control interaction with p300 and

transcription factors through multivalent interaction after DNA damage.