Page 2 - V. Proteomics
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[V. Proteomics-2]
Proteomic profiling of CYP11A1 overexpressed kidney cancer
cell line
Hien Thi My Ong¹˙², Min-Jung Kang¹˙²˙*,
¹Molecular Recognition Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5,
Seongbuk-gu, Seoul 136-791, Republic of Korea, ²Division of Bio-Medical Science & Technology, KIST School,
Korea University of Science and technology, Seoul 02792, Republic of Korea
Our study focused on the comparative proteomic profiling of CYP11A1 overexpressed normal and kidney cancer
cell lines. Kidney cancer is the 12th cause of death from cancer worldwide which is currently showed a poor
prognosis. Molecular mechanism study of kidney cancer cell including protein and gene expression profiles as
potential novel prognostic biomarkers could provide a clue to discover cancer biomarker specific for kidney cancer.
CYP11A1 catalyze the initial step in the conversion of cholesterol to pregnenolone and associated with various
cancer types. In this context, two types of kidney cancer (A498 and Caki1) and normal kidney cell (HEK293) were
transfected with CYP11A1. Elevated expression of CYP11A1 was detected and quantified by western blotting. Cell
lysates were analyzed by nanoLC-MS/MS and identified by mass spectrometry searched using Proteome Discoverer
2.3 against the NCBI database. We optimized various conditions for transfection, trypsin digestion and peptide
analysis method. After comparing mass spectrometry data with Interpretative Phenomenological Analysis, several
proteins were discovered which expression levels are associated with kidney cancer. The mechanism study of
signaling pathway for CYP11A1 synthesizing steroid hormones will be studied further.
