Page 53 - Q. Neuroscience

Alterations of intestinal neurotrophins in neurotoxin-induced

Parkinson’s disease mouse model
b
Jin Gyu Choi a,b,c,1 , Ji-Hye Ahn a,b,d,1 , Miran Jeong ,a,b,c , Boh Rah Joo , Jeong-Hwa Woo , Dong-Hyun Kim a,b,c ,
b
Myung Sook Oh a,b,c,* , Jung-Hye Choi a,b,c,*
a Neurobiota Research Center (NRC), Kyung Hee University, Seoul 02447, South Korea
b College of Pharmacy, Kyung Hee University, Seoul 02447, South Korea
c Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 02447, South Korea
d College of Pharmacy, Woosuk University, Jeonbuk 55338, South Korea
1 These authors contributed equally to this study. *Corresponding authors
BACKGROUND AIM
- Emerging evidence suggests that intestinal neurotrophins play a In this study, we investigated the levels of 4
key role in the gut-brain axis, a bidirectional communication neurotrophins (neurotrophin A-D) in the intestine
between the gut and the brain. (small and large) of 1-methyl-4-phenyl-1,2,3,6
- Alternations of gut neurotrophins have been shown in several tetrahydropyridine (MPTP)-induced PD mice.
neurological disorders, but a remarkable change of gut
neurotrophins in Parkinson’s disease (PD) animal models remains
unexplored.

METHODS
- MPTP-induced PD mouse model - Measurement of the mRNA levels
using quantitative reverse transcription PCR (RT-qPCR)

- Measurement of the protein levels
using western blotting and ELISA kit

RESULTS

CONCLUSION ACKNOWLEDGEMENTS

These findings suggest that the significant alteration of This study was supported by Medical Research Center
intestinal neurotrophin A expression observed in Program through the National Research Foundation of Korea
neurotoxin-induced PD animal model may play a role in funded by the Ministry of Science and ICT (NRF-
the gut-brain axis in PD and be a promising marker for 2017R1A5A2014768).
diagnosing PD.