Page 28 - O. Microbiology

[O. Microbiology-22]

Comparative microbial communities of tonsils and saliva in

children subjected to tonsillectomy

Da Hyeon Choi¹˙#, Jiwon Park¹˙#, Ju Kwang Choi¹, Kyeong Eun Lee¹, Won Hee Lee², Jinho Yang², Chan Oh³,
Ho-Ryun Won³, Bon Seok Koo³, Jae Won Chang³˙*, Yoon Shin Park¹˙*

¹Microbiology, Chungbuk National University, Cheongju 28644, Republic of Korea, ²-, MD Healthcare Inc., Seoul -,

Republic of Korea, ³Otolaryngology-Head and Neck Surgery, Chungnam National University, Daejeon 35015,
Republic of Korea

Oral bacteria travel throughout the body and are significantly associated with human diseases. Given that tonsils

are located between the oral cavity and larynx esophagus at the gateway of both alimentary and respiratory tracts,
tonsillar tissue may also be affected by both oral and alimentary tract microbiota. Here, we focused on the

distribution and correlations of microbiota in saliva and tonsillar tissues of young tonsillectomy patients based on
evaluation of the V3-V5 region of 16S rRNA genes to examine the hypothesis that the microbiome is associated

with tonsillar hyperplasia in children. The top 10 ranked taxa in saliva group and tonsillar group based on average
relative abundance were Haemophilus, Streptococcus, Fusobacterium, Veillonella, Prevotella, Alloprevotella, Neisseria,

Prophyromonas, Campylobacter, and Treponema 2. Analysis of the microbiomes between tonsil and saliva revealed
that many bacterial communities are shared and show similarities in terms of diversity and composition, suggesting

close interactions between the two microbial groups. Our results assume that the oral microbiome exerts significant
effects on not only the tonsil itself but also tonsil-derived immune or stem cells through regulating the microbial

community.