Page 17 - O. Microbiology

Roles of Herp in mycobacterial infected antigen presenting cells

Sang-Hun Son1,2, Ji-Ae Choi1,2, Junghwan Lee1,2, Soo-Na Cho1,2 , Nguyen Tam Doan1,2, Seongahn Lee1,2 and Chang-Hwa Song1,2,3
1 Department of Medical Science, 2 Department of Microbiology and 3 Translational immunology institute, College of Medicine, Chungnam National University,
Daejeon 35015, South Korea

BACKGROUND AIM

Endoplasmic reticulum (ER) stress plays an important role in the We found that herp was strongly increased in macrophage by
pathogenesis of tuberculosis. The homocysteine-inducible ER Mycobacterium tuberculosis (Mtb) H37Ra infection. ER stress
regulates Herp production during Mtb infection. Herp depletion
stress protein (Herp), part of the ER-associated protein increases reactive oxygen species production via ER stress
degradation (ERAD) pathway molecules, has not been known as regulation. Inhibition of Herp expression by using specific siRNA
a anti-mycobacterial factor. induced the LC3 and decreased p62, resulting in decrement of
intracellular survival of Mtb.
METHODS

To explore the role of Herp in tuberculosis, we compared suppressive effect of mycobacterial growth between Herp knockout
Raw264.7 cells and wild type control after mycobacterial infection.

RESULTS

CONCLUSION REFERENCES ACKNOWLEDGEMENTS

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