Page 2 - K. Development and regeneration

[K. Development and regeneration-1]

Expression pattern and developmental function of Piezo1 in

tooth and salivary glands during embryogenesis

Eui-Seon Lee¹, Yam Prasad Aryal¹, Tae-Young Kim¹, Elina Pokharel¹, Shijin Sung¹, Ji-Youn Kim², Wern-Joo Sohn³,
Sung-Jin Cho⁴, Jae-Kwang Jung¹, Jae-Young Kim¹˙*

¹School of Dentistry, Kyungpook National University, Daegu 41944, South Korea, ²Department of Dental Hygiene,

Gachon University, Incheon 21936, South Korea, ³Pre-Major of Cosmetics and Pharmaceutics, Daegu Haany
University, Gyeongsan 38610, South Korea, ⁴School of Biological Sciences, Chungbuk National University,
Cheongju 28644, South Korea

Recent researches showed the importance of mechanically-activated factors in condition of organogenesis,
especially in formation of hard tissue and secretory organs. Craniofacial regions are composed of harmonious

patterned arrangement of hard and secretory tissues in restricted area. Piezo type mechanosensitive ion channel
component 1 (Piezo1) is a sensor and transducer of mechanical stimuli which has critical roles in various mechano-

transduction processes in touch, pain, proprioception, vascular development and blood pressure regulation. In this
study, we examined the detail expression pattern of Piezo1 using in situ hybridization and RT-qPCR. To understand

the precise signaling regulations of Piezo1, we evaluated the related signaling network, we examined the altered
expression patterns of signaling molecules after treatment of siRNA Piezo1 (siPiezo1) using RT-qPCR. Piezo1

downregulated SMG using siPiezo1 with in vitro organ cultivation was histologically observed with
immunohistochemistry. Our result suggests that Piezo1 modulates mechanosensitive molecules related with nerve

innervation, in hard tissue: pulp-dentin complex and in gland: acinar cells and duct forming regions.