Page 36 - I. Chemical biology and drug discovery

[I. Chemical biology and drug discovery-19]

Increasing protein self assembly through Ni(II)-nitrilotriacetic

acid system from novel water soluble ligands

Hee-Kwon Kim¹˙*

¹Department of Nuclear Medicine, Jeonbuk National University , Jeonju 54907, Republic of Korea

It was reported that nitrilotriacetic acid(NTA) can interact with Ni(II), and nitrilotriacetic acid(NTA) technology is able
to have selective and high binding affinity to Histidine-tagged proteins. Ni(II)-Nitrilotriacetic acid system were
applied to several biological study including protein purification, and fluorescence labeling. For protein crystallization,

the initial crucial step is protein nucleation. Therefore, management of protein nucleation process is crucial for rate

of protein crystallization. In the present study, various novel ligands containing multivalent nitrilotriacetic acids were
designed to check the possible to increase of protein crystallization. These newly prepared ligands were prepared
via coupling reaction of core structure from commercially available reagents. These ligands have symmetric structure,

and showed water-soluble properties. In vitro experiment using green fluorescent protein (GFP) for the treatment
of ligands, biological analysis data showed that larger size of complexes of GFP protein:ligand was generated due

to intermolecular interactions, suggesting that new ligands assists nucleation of protein. Our results indicate that
protein nucleation step can be enhanced via the interaction of novel water soluble chelating agents.