Page 10 - H. Cell signaling

[H. Cell signaling-5]

Analysis of melanin synthesis inhibitor from Butanol fraction

of Ligustrum lucidum Ait

Chae-Rin Kim¹˙#, Ji-Eun Park¹˙#, Ga-Eun Yoo¹, Hae-Ryeong Lim¹˙*, Deug-Chan Lee¹˙*

¹Department of Medical biotechnology, College of Biomedical Science, Kangwon National University, Chuncheon-

si, Gangwon-do 200-701, Republic of Korea

Ligustrum lucidum Ait is a fruit of Ligustrum japonicus. For many decades, Ligustrum lucidum Ait is used for medical

purpose, especially for liver and kidney, in Asia. Also, biological effects of Ligustrum lucidum Ait. such as antidiabetic
and antioxidant have been studied. 70% ethanol extract of dried Ligustrum lucidum Ait showed inhibitory effect on
melanin synthesis using mouse melanocyte, B16F10 cells. 70% ethanol extract was fractionated to various solvents

(hexane, ΔDichloromethane, Ethyl acetate, butanol, aqueous solvent). Butanol fraction produced highest yield and
extremely inhibited melanin synthesis than other fractions