Page 7 - H. Cell signaling

p32 conditional knock out of endothelium was increased
vascular function via activation of CaMKll/AMPK/p38
MAPK/Akt/eNOS axis

Byeongjun Yoon and Sungwoo Ryoo
Department of Biology, College of Natural Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea

ABSTRACTS INTRODUCTIONS
● The decrease in expression of p32 induced the activity of the CaMKll/AMPK ● Ca 2+ is a major secondary messenger and can induce various cellular
2+
/p38 MAPK/Akt/eNOS signaling pathway responses, which can activate Ca /calmodulin-dependent protein kinasell
(CaMKll) and endothelial nitric oxide synthase (eNOS).
● p32 conditional knock out (cKO) increased the activity of eNOS in a Ca -
2+
dependent manner, which increased NO production ● eNOS produces nitric oxide (NO) using L-arginine as a substrate and NO
plays important role in vascular homeostasis.
+
● In the aorta of p32 Tie Cre mice, the acetylcholine relaxation response was
f/f
increased and the phenylephrine contraction response was decreased. ● p32 has a donut-shaped homotrimer structure with a pore in the center,
which acts as a Ca 2+ channel and can regulate cytosolic Ca .
2+
2+
● In conclusion, p32 can regulate the cytosolic Ca , which can induce eNOS
activity and improve vascular function. ● Since cytosolic Ca 2+ can regulate eNOS activity, p32 may be associated
with eNOS
RESULTS

Fig. 1. Generate design of p32 f/f Tie Cre + mice and p32 is conditional Fig. 2. p32 cKO increased cytosol Ca 2+ Fig. 3. p32 cKO increased phospholyation
knock out of endothelium level in MLEC of CaMKll/AMPK/p38 MAPK/Akt/eNOS.

Supplementary

Fig. 4. Increased eNOS activity induced NO production, which increased vascular Fig. 1. Blood pressure tended to decrease, but
reactivity. there was no significance.
CONCLUSION REFERENCES

2+
● p32 can increase the activity of eNOS in a Ca -dependent manner, which ● Koo, B. H., Hwang, H. M., Yi, B. G., Lim, H. K., Jeon, B. H., Hoe, K. L., ... &
can increase the reactivity of blood vessels. Ryoo, S. (2018). Arginase II Contributes to the Ca2+/CaMKII/eNOS Axis by
Regulating Ca2+ Concentration Between the Cytosol and Mitochondria in a
p32‐Dependent Manner. Journal of the American Heart Association, 7(18),
● Further research is needed on the mechanism by which p32 regulates e009579.
cytosolic Ca 2+.
● Yagi, M., Uchiumi, T., Takazaki, S., Okuno, B., Nomura, M., Yoshida, S. I., ...
& Kang, D. (2012). p32/gC1qR is indispensable for fetal development and
mitochondrial translation: importance of its RNA-binding ability. Nucleic
acids research, 40(19), 9717-9737.