Page 34 - G. Cell differentiation. division. and death

[G. Cell differentiation, division, and death-22]

Identification of Novel Biomarker and Signaling pathways

Associated with Interstitial Cystitis/Bladder Pain Syndrome

for Better Diagnosis

Subbroto Kumar Saha¹˙#, Tak Il Jeon¹˙#, Soo Bin Jang¹˙#, Yu Jin Choi¹, Se Jong Kim¹, Kyung Min Lim¹, Hee Jeong

Kwak¹, Yoon Joo Lee¹, Jong Yub An¹, Han Bit Bong¹, Hyeong Gon Kim², A ram Kim²˙*, Ssang Goo Cho¹˙*

¹Stem Cell and Regenerative Biotechnology, Konkuk University, SEOUL 05029, 대한민국, ²Urology, Konkuk

University Medical Center, Konkuk University School of Medicine, SEOUL 05029, 대한민국

The complexity of interstitial cystitis/bladder pain syndrome (IC/BPS) has led to considerable uncertainty in terms
of diagnosis and prevalence of the condition. Here, we try to identify the IC/BPS-associated genes through an

integrated analysis of Gene Expression Omnibus datasets and confirm experimentally to predict the pathologic
diagnosis of IC/BPS. Data mining analysis of GEO datasets revealed a total of 53 common differentially expressed

genes in IC/BPS. A protein–protein interaction network was then constructed with the 53 common DEGs using
Cytoscape v3.7.2, and next, six hub genes (CD5, CD38, ITGAL, IL7R, KLRB1, and IL7R) were identified using cytoHubba

v0.1 that were upregulated in IC/BPS. Enrichment analysis of common DEGs revealed that hematopoietic cell lineage,
immune system, and T-cell receptor signaling in naïve CD4+ T cell signaling pathways were prominently involved

with the common 51 upregulated DEGs. Moreover, our RT-PCR data confirmed that the expression of the five hub
genes (CD38, ITGAL, IL7R, KLRB1, and IL7R) was significantly augmented in IC/BPS patients’ samples when compared

with their normal counterparts. In this study, we systematically predict the significant biomarkers and possible
signaling pathways involved in IC/BPS.