Page 3 - C. Biotechnology and molecular imaging

Auto-inducible, dynamic, and antibiotic-free expression system in Escherichia
coli for the production of farnesol
1
Mst. Fayzunnesa , and Seon-Won Kim 1
1 Division of Applied Life Science (BK21 plus Program), PMBBRC, Gyeongsang National University, Jinju
52828, Republic of Korea
Background AIM
Engineered E.coli MG1655 strain overexpressing heterologous MVA The main purpose of this study is to
pathway genes or along with native MEP, pathway produces IPP and establish an auto-inducible and dynamic
DMAPP buildingblocks for FPP synthesis catalyzed by FPP synthase system in E.coli for the production of
(IspA). Subsequently, FPP could then be depyrophosphorylated to Farnesol in an economic way.
form farnesol via NudJ, an E.coli native phosphatase suspected to
dephosphorylate IPP and DMAPP to isoprenoid alcohols. To establish Secondly, the purpose of the study is to
an auto-inducible and dynamic system IspA, idi, and MVA pathway introduce an antibiotic-free bio-culture
genes were overexpressed under the control of inducer-free promoters system in E. coli for the production of
PgadE and PrstA successfully producing the targeted products farnesol on a large scale and also accost
farnesol for 48 hours. effective manner.
METHODS

Construction of these following plasmid and then culture them for production

RESULTS

Figure 1. Cell growth and Production improvement by Figure 2 Large scale production of Farnesol
using an antibiotic-free plasmid-based system comparing with using in E. coli MG1655 with antibiotic and
an inducer-free system in E. coli MG1655. inducible plasmid based system comparing
antibiotic-free system

CONCLUSION REFERENCES ACKNOWLEDGEMENTS

Farnesol can be a very [1] Wang, Chonglong, et al. "Metabolic This work was supported by a grant
promosing candidate for engineering and synthetic biology approaches from the Next-Generation BioGreen 21
advanced biofuel and driving isoprenoid production in Escherichia Program (SSAC, grant#: PJ01326501),
commodity chemicals. coli." Bioresource technology 241 (2017): 430- Rural Development Administration,
Republic of Korea.
438.
Introducing Some inducer-free [2] Zada, Bakht, et al. "Metabolic engineering of
and Antibiotic-free expression Escherichia coli for production of mixed Contact information
system farnesol can be isoprenoid alcohols and their
produced in a successful way. derivatives." Biotechnology for biofuels 11.1 E-mail: swkim@gnu.ac.kr
(2018): 210. E-mail: mst.fayzunnesa@yahoo.com
.