Page 196 - ebook

[B. Cell Biology/Stem Cell] B-33

The advanced therapeutic effect of MY145-enhanced

mesenchymal stem cells on functional recovery and

neurogenesis in stroke model

Hye Ryeong Sim¹, Jee In Choi¹,², Hyun-Jin Kim¹, Min Young Kim¹,²*

¹Department of Rehabilitation Medicine, CHA Bundang Medical Center, CHA University School of Medicine,

Seongnam 13496, Korea, ²Rehabilitation and Regeneration Research Center, CHA University School of Medicine,

Seongnam 13488, Korea

Ischemic stroke had limited treatment options due to the irreversibility of neuronal tissue damage. Recent studies
suggest stem cell based therapy as a promising treatment for stroke; however, its therapeutic effect is insufficient.

In our previous clinical study, we found that MY145, a marker of efficacy, was increased in protein screening after
stem cell therapy. The aim of this study was to investigate the role of MY145 and to elucidate the effect of MY145

enhancing the function of stem cells. The Modified neurological severity score (mNSS) results showed that MY145-
transfected stem cell further improved the neurological impairment compared to stem cell. In addition, Neuronal

nuclei (NeuN) and doublecortin (DCX), the expression of neurogenesis markers, were increased by all treatments
and upregulated most by MY145-transfected stem cell therapy. To confirm the role of MY145, we measured cell
proliferation and MY145 mRNA levels after stem cell co-culture of OGD/R-induced bEnd.3 cell. Also, stem cell

therapy significantly upregulated mRNA levels of Vascular Endothelial Growth Factor (VEGF) and basic Fibroblast

Growth Factor (bFGF), which are angiogenesis markers. In conclusion, this study suggests that MY145-enhaced MSC
has synergistic effects and that MY145 is a potential marker for predicting the therapeutic effect of stem cell therapy.