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P. 142
[B. Cell Biology/Stem Cell] B-6
The Oxysterols 27-hydroxycholesterol influences pools of
hematopoietic stem and progenitor cells
Hee Seon Choi¹, Jayoung Kim¹, Soo-Yeon Woo¹, Munju Kwon¹, Dongjun Lee¹*
¹Department of Convergence medicine, Pusan national university, Pusan 50612, Korea
Oxysterols are oxygenated derivatives of cholesterol and, compared to cholesterol, contain an additional hydroxy,
epoxide or ketone group in the sterol nucleus, and/or a hydroxyl group in the side chain. 27-hydroxycholesterol
(27HC) is a side-chain oxysterol oxygenated at the 27 the carbon atom of cholesterol. This oxysterol is produced
via oxidation by sterol 27-hydroxylase (CYP27A1) and metabolized via 7α-hydroxylation for bile acid synthesis in
the liver. The previous study demonstrated that treatment with the alternative ERα ligand 27HC induced ERα-
dependent HSC mobilization. Also, Cyp27a1-deficient mice had significantly reduced 27HC levels and HSC
mobilization. In the present study, we investigated whether 27HC affected HSC functions. Here, we report that 27HC
exhibit impaired hematopoietic stem and progenitor cell (HSPC) numbers, but did not influence mature cell output
in vitro. Furthermore, 27HC has increased ROS levels from HSPCs. Thus, 27HC is indispensable for regulating pools
of hematopoietic stem and progenitor cells.
