Page 112 - ebook

[A. Biochemistry/Molecular Biology] A-56

Ethanol extract of Mahkota Dewa (Phaleria macrocarpa)

protects UVB-induced skin photoaging damage in human

keratinocytes by suppressing MMP expression

Yeo Won Jeoung¹, Ye Jin Jang², Yunok Oh³*

¹Department of Cosmeceutical Science, Daegu Haany University, Daegu 38578, Korea, ²Department of Cosmetic

Science, Kyungsung University, Busan 48434, Korea, ³Convergence Research Center for SmartHealthcare,

Kyungsung University, Busan 48434, Korea

The edible fruits of Mahkota dewa (Phaleria macrocarpa) are frequently used in traditional medicine, such as cancer,
allergies, diabetes mellitus, heart diseases, and skin diseases. Despite Mahkota dewa’s well-known benefits, its use

in preclinical studies is still limited due to poisonous properties. Therefore, it is of great interest to ascertain the
biochemical and cytotoxic properties of the fruit extracts of Mahkota dewa. In this study, the ethanol (70%) and

water extract of Mahkota Dewa were evaluated for protection of UVB-induced skin photoaging. The ethanol extract
of Mahkota Dewa (MDE) displayed high antioxidant activity on DPPH (IC50= 50.2 μg/mL) and ABTS (IC50= 27.8

μg/mL) assay compared to that of the standard ascorbic acid. We next investigated skin protection effects of MDE
in HaCaT keratinocyte cells. Our results showed that MDE with 100 g/mL had no cytotoxic effect and showed
cytoprotective effect UVB-induced cell damage in HaCaT cells via inhibition of intracellular ROS production and

enhanced Nrf2- nuclear accumulation. MDE also suppressed collagen degradation in HaCaT cells via down-

regulation of MMP expression. The results suggest that MDE offer antioxidant activity and anti-wrinkle effect against
UVB-induced photoaging damage in human skin fibroblasts.