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[B. Cell Biology/Stem Cell] B-25

Ovarian Cancer Stem cells targeted anti-CD44 conjugated,

and cisplatin loaded PLGA nanoparticles on tumor associated

macrophages embedded 3D spheroid models

Samjhana Shrestha¹, Simmyung Yook¹*

¹Pharmacy, Keimyung University, Daegu 대구광역시 달서구 달구벌대로, Korea

Background: The presence of cancer stem cells (CSCs) and their interplay with tumor associated macrophage (TAM)
have made ovarian cancer (OC) drug resistant toward Cisplatin (Cis). CSC surface marker CD44 targeted therapy

using PLGA nanoparticles can be an attractive therapeutic strategy for treatment of recurrent OC. In this study, since
3D culture spheroids models can mimic the OC tumor microenvironment, we developed 3D cancer model in the

presence of TAM and evaluated the effect of CD44 targeted and Cis-loaded PLGA nanoparticles (anti-CD44-Cis-
PLGA NPs) for treatment of recurrent OC. Methods: Cis was loaded in PLGA with double emulsion solvent

evaporation procedure. The formulation is characterized by average particle size, zeta potential and polydispersity
(PDI), loading capacity (LC) and encapsulation efficiency (EE). The surface modification of Cis-loaded PLGA was done

by EDC/NHS chemistry and is characterized by pierce bichinchoninic acid (BCA) protein assay. Results: The average
particle size and PDI of Cis-loaded PLGA were119.8 ±1.84 nm and 0.14 ± 0.02, respectively. The LC and EE were
5.55 ± 0.12 % and 18.5 ± 0.417% respectively. Conclusion: Our results demonstrated that the Cis-PLGA NPs were

successfully constructed, and this targeted nano system can be a potential candidate for treatment of recurrent OC.