Page 44 - ebook

[A. Biochemistry/Molecular Biology] A-22

Effects of Saposhnikovia divaricata (Turcz.) Schischk extract

on inflammation and gut microbial composition in DSS-

induced colitis

Saruul Erdenebileg¹,², Yang-Ju Son³, Myungsuk Kim¹, Da Seul Jung¹, Yunseong Nam¹, Nadzeya Lazerka¹,

Chu Won Nho¹,²*

¹Smart Farm Research Center, Gangneung Institute of Natural Products Korea Institute of Science and Technology

(KIST), Gangneung, Gangwon-do 25451, Korea, ²Division of Bio-Medical Science and Technology, KIST School,
Korea University of Science and Technology (UST), Daejeon 34113, Korea, ³Department of Food and Nutrition,

Chung-Ang University, Anseong-si, Gyeonggi-do 17546, Korea

Inflammatory Bowel Disease (IBD), chronic inflammation in the gastrointestinal tracts, affects up to 1 in 200
individuals per year. Most current clinical drugs for IBD lead to research for new possible treatments due to their

adverse effects. Root of Saposhnikovia divaricata (Turcz.) Schischk (SD) has been used as a medicinal herb with its
anti-inflammatory and analgesic effects. In this study, we investigated the effect of SD extracts on inflammation and

gut microbiota modulation in vitro and colitis-induced mice. We purified seven compounds from methanol extract
of SD roots (MESD), and found MESD and two compounds; deltoin and 3-O-angeloylhamaudol, reduced level of

nitric oxide in Raw 264.7 mouse macrophages. Oral administration of MESD lowered pro-inflammatory cytokines,
IL-1β and TNFα, in the serum and the colon in dextran sulfate sodium (DSS)-induced IBD mouse model. Furthermore,

MESD significantly shifted the gut microbial composition. DSS treatment significantly increased the abundance of
pathogens and decreased the butyrate producing bacteria. MESD decreased the abundance of genera Clostridium

sensu stricto 1 and enriched some of predicted metabolic activities such as chondroitin sulfate degradation. These
results suggest that MESD may ameliorate the DSS-induced colitis inflammation by regulating the compositions

and functions of gut microbiota in mice.